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Brefeldin A (Cyanein) 属于大环内酯类抗生素,是一种 ATPase 抑制剂 (IC50=0.2 μM)。Brefeldin A 可以诱导肿瘤细胞分化和凋亡,也具有抑制自噬的活性。
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Brefeldin A (Cyanein) 属于大环内酯类抗生素,是一种 ATPase 抑制剂 (IC50=0.2 μM)。Brefeldin A 可以诱导肿瘤细胞分化和凋亡,也具有抑制自噬的活性。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
5 mg | ¥ 414 | In stock | |
10 mg | ¥ 698 | In stock | |
50 mg | ¥ 2,470 | In stock | |
100 mg | ¥ 3,859 | In stock | |
1 mL x 10 mM (in DMSO) | ¥ 414 | In stock |
Brefeldin A 相关产品
产品描述 | Brefeldin A (Cyanein) belongs to the class of macrolide antibiotics and is an ATPase inhibitor (IC50=0.2 μM). Brefeldin A can induce tumor cell differentiation and apoptosis, and also possesses autophagy inhibitory activity. |
靶点活性 | ATPase (HCT 116 cells):0.2 μM, A549 cells proliferation:101.2 nM, HeLa cells proliferation:171.9 nM, HepG2 cells proliferation:239.1 nM |
体外活性 | 方法:肿瘤细胞 HL60 、K562 和 HT-29 用 Brefeldin A (2 μM) 处理 72 h,使用 DNA filter elution assay 检测 DNA 片段。 |
体内活性 | 方法:为检测体内抗肿瘤活性,将 Brefeldin A (15 mg/kg in 10% ricinus oil+5% DMSO+10% ethanol+75% physiologic saline) 腹腔注射给携带人结直肠癌肿瘤 HCT116 的 BALB/c 小鼠,每天一次,持续四周。 |
激酶实验 | ELISA-based active site binding assay: Samples (lysed cells or tissue homogenates) are treated for 1 h at room temperature with the biotinylated active site probe PR-584 (5-15 μM). Samples are denatured by addition of SDS (0.9% final) and heating to 100 °C for 5 min. The denatured samples are transferred to a 96-well or 384-well filter plat, mixed with streptavidin-sepharose beads (2.5-5 μL packed beads/well), and incubated for 1 h at room temperature on a plate shaker. The beads are washed 5 times with 100-200 μL /well of ELISA buffer (PBS, 1% bovine serum albumin, 0.1% Tween-20) by vacuum filtration. The beads are incubated overnight at 4 °C on a plate shaker with the following antibodies recognizing the six catalytic subunits diluted into ELISA buffer: β5, β1, and β2 diluted 1:3000, LMP7 and LMP2 diluted 1:5000, and MECL-1 diluted 1:1000. The beads are washed 5 times with 100-200 μL /well of ELISA buffer and incubated with HRP-conjugated secondary antibody diluted 1:5000 in ELISA buffer and incubated 2 h at room temperature on a plate shaker. The beads are washed 5 times with 100-200 μL /well of ELISA buffer and developed for chemiluminsecence signal using the supersignal ELISA pico substrate following the manufacturer's instructions. Luminescence is measured on a plate reader and converted to ng of proteasome or μg/ml of lysate by comparison with 20S proteasome or untreated cell lysate standard curves. For proteasome inhibitor studies, active site probe binding values are expressed as the percent of binding relative to DMSO treated cells. |
细胞实验 | HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.(Only for Reference) |
别名 | 布雷菲德菌素 A, 布雷非德菌素 A, Decumbin, Cyanein, BFA, Ascotoxin |
分子量 | 280.36 |
分子式 | C16H24O4 |
CAS No. | 20350-15-6 |
Smiles | [H][C@@]12C[C@H](O)C[C@@]1([H])[C@H](O)\C=C\C(=O)O[C@@H](C)CCC\C=C\2 |
密度 | 1.108 g/cm3 (Predicted) |
存储 | store at low temperature,keep away from moisture,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
溶解度信息 | Ethanol: 2.8 mg/mL (9.99 mM), Sonication is recommended. DMSO: 100 mg/mL (356.68 mM), Sonication is recommended. ![]() | ||||||||||||||||||||||||||||||||||||||||
溶液配制表 | |||||||||||||||||||||||||||||||||||||||||
Ethanol/DMSO
DMSO
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以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。
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